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验证数据展示

  • WB analysis of PC-3 using 25447-1-AP

    WB analysis of PC-3 using 25447-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25447-1-AP (C15orf58 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 25447-1-AP

    IF Staining of PC-3 using 25447-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 25447-1-AP (C15orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

C15orf58 Polyclonal antibody

C15orf58 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25447-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

C15orf58, UPF0580 protein C15orf58

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, mouse brain tissue
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25447-1-AP targets C15orf58 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C15orf58 fusion protein Ag20215 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 15 open reading frame 58
别名 C15orf58, UPF0580 protein C15orf58
计算分子量 385 aa, 42 kDa
观测分子量 45 kDa
GenBank蛋白编号BC127821
基因名称 C15orf58
Gene ID (NCBI) 390637
RRIDAB_2880085
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZNW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C15orf58 antibody 25447-1-APDownload protocol
IF protocol for C15orf58 antibody 25447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols