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验证数据展示

  • WB analysis using 28639-1-AP

    WB analysis using 28639-1-AP

    Mouse liver tissue were subjected to SDS PAGE followed by western blot with 28639-1-AP (C15orf55 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 28639-1-AP

    IHC staining of mouse testis using 28639-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28639-1-AP (C15orf55 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 28639-1-AP

    IF Staining of A431 using 28639-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C15orf55 antibody (28639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

C15orf55 Polyclonal antibody

C15orf55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28639-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IHC, IF/ICC, ELISA

别名

C15orf55, DKFZp434O192, Nuclear protein in testis, NUT, Protein NUT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse testis tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28639-1-AP targets C15orf55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原

CatNo: Ag29239

Product name: Recombinant human C15orf55 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 496-783 aa of BC114567

Sequence: SGSVEDEDGDGRLRPSPGLQGAGGAACLGKVSSSGKRAREVHGGQEQALDSPRGMHRDGNTLPSPSSWDLQPELAAPQGTPGPLGVERRGSGKVINQVSLHQDGHLGGAGPPGHCLVADRTSEALPLCWQGGFQPESTPSLDAGLAELAPLQGQGLEKQVLGLQKGQQTGGRGVLPQGKEPLAVPWEGSSGAMWGDDRGTPMAQSYDQNPSPRAAGERDDVCLSPGVWLSSEMDAVGLELPVQIEEVIESFQVEKCVTEYQEGCQGLGSRGNISLGPGETLVPGDTES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 15 open reading frame 55
别名 C15orf55, DKFZp434O192, Nuclear protein in testis, NUT, Protein NUT
计算分子量 1132 aa, 120 kDa
观测分子量 150 kDa
GenBank蛋白编号BC114567
基因名称 C15orf55
Gene ID (NCBI) 256646
RRIDAB_2881186
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86Y26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C15orf55 antibody 28639-1-APDownload protocol
IHC protocol for C15orf55 antibody 28639-1-APDownload protocol
IF protocol for C15orf55 antibody 28639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols