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验证数据展示

  • WB analysis using 30664-1-AP

    WB analysis using 30664-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30664-1-AP (C15orf42 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30664-1-AP

    IF Staining of A431 using 30664-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C15orf42 antibody (30664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TICRR Polyclonal antibody

TICRR Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30664-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

C15orf42, TopBP1-interacting checkpoint and replication regulator, TopBP1-interacting, replication-stimulating protein, Treslin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30664-1-AP targets TICRR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33632

Product name: Recombinant human C15orf42 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 241-590 aa of BC033209

Sequence: VELEMQASGLPKLRIKKIDPSSSLEAEPLSKEESSLGEESFLPALSMPRASRSLSKPEPTYVSPPCPRLSHSTPGKSRGQTYICQACTPTHGPSSTPSPFQTDGVPWTPSPKHSGKTTPDIIKDWPRRKRAVGCGAGSSSGRGEVGADLPGSLSLLESEGKDHGLELSIHRTPILEDFELEGVCQLPDQSPPRNSMPKAEEASSWGQFGLSSRKRVLLAKEEADRGAKRICDLREDSEVSKSKEGSPSWSAWQLPSTGDEEVFVSGSTPPPSCAVRSCLSASALQALTQSPLLFQGKTPSSQSKDPRDEDVDVLPSTVEDSPFSRAFSRRRPISRTYTRKKLMGTWLEDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 15 open reading frame 42
别名 C15orf42, TopBP1-interacting checkpoint and replication regulator, TopBP1-interacting, replication-stimulating protein, Treslin
观测分子量210-250 kDa
GenBank蛋白编号BC033209
基因名称 C15orf42
Gene ID (NCBI) 90381
RRIDAB_3669748
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z2Z1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C15orf42 (TOPBP1 interacting checkpoint and replication regulator), also known as Treslin. It is expected to be located in cytosol and nucleoplasm, the protein is mainly expressed in bone marrow, esophagus, and lymphoid tissue. The protein C15orf42 enables chromatin binding activity and it is involved in regulation of DNA-dependent DNA replication initiation. And the calculated molecular weight of C15orf42 is 210 kDa, the 250 kDa band has been reported in the literature (PMID: 30037903).

实验方案

Product Specific Protocols
IF protocol for TICRR antibody 30664-1-APDownload protocol
WB protocol for TICRR antibody 30664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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