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  • Featured Product
  • KD/KO Validated

C14orf39 Polyclonal antibody

C14orf39 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 22664-1-AP

Print datasheet

Synonyms

C14orf39, Six6os1

验证数据展示

  • WB analysis of Jurkat using 22664-1-AP

    WB analysis of Jurkat using 22664-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22664-1-AP

    IHC staining of human skeletal muscle using 22664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

  • IHC staining of human skeletal muscle using 22664-1-AP

    IHC staining of human skeletal muscle using 22664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 22664-1-AP

    IF Staining of MCF-7 using 22664-1-AP

    Immunofluorescent analysis of MCF-7 cells using 22664-1-AP (C14orf39 antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse colon tissue, SKOV-3 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22664-1-AP is C14orf39 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen C14orf39 fusion protein Ag18589 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 39
Synonyms C14orf39, Six6os1
Calculated Molecular Weight 587 aa, 68 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC125070
Gene Symbol C14orf39
Gene ID (NCBI) 317761
RRIDAB_2879146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1H7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C14orf39 antibody 22664-1-APDownload protocol
IHC protocol for C14orf39 antibody 22664-1-APDownload protocol
IF protocol for C14orf39 antibody 22664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility.

Authors - Laura Gómez-H
  • KO Validated
View Article