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验证数据展示

  • WB analysis of Jurkat using 22664-1-AP

    WB analysis of Jurkat using 22664-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22664-1-AP (C14orf39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22664-1-AP

    IHC staining of human skeletal muscle using 22664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

  • IHC staining of human skeletal muscle using 22664-1-AP

    IHC staining of human skeletal muscle using 22664-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22664-1-AP (C14orf39 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 22664-1-AP

    IF Staining of MCF-7 using 22664-1-AP

    Immunofluorescent analysis of MCF-7 cells using 22664-1-AP (C14orf39 antibody) at dilution of 1:50.

  • IF Staining of mouse testis using 22664-1-AP

    IF Staining of mouse testis using 22664-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using C14orf39 antibody (22664-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

SIX6OS1 Polyclonal antibody

SIX6OS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22664-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

C14orf39, Protein SIX6OS1, Six6 opposite strand transcript 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse colon tissue, SKOV-3 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22664-1-AP targets SIX6OS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag18589

Product name: Recombinant human C14orf39 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-587 aa of BC125070

Sequence: ETLEEKNKNTENRKELKERIFGKDEHVLTLNKTQSSQLFLPYESQKLVRPIKMHSSEPRVADIKEESSAKQSKLANIDFRQKENDTQIFNDSAVDNHSKCSHITTITSSQKFMQVRLLTPQKQSNSNQWSEKGDKDAEYGDKGTVRQVRESKCTSQAIYTEHFGKSIENDSDEVEERAENFPRTSEIPIFLGTPKAVKAPESLEKIKFPKTPPFEINRNRNAVPEVQTEKESPGLSFLMSYTSRSPGLNLFDSSVFDTEISSDQFNEHYSARNLNPLSSEQEIGNLLEKPEGEDGFTFSFPSDTSTHTFGAGKDDFSFPFSFGQGQNSIPSSSLKGFSSSSQNTTQFTFF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 39
别名 C14orf39, Protein SIX6OS1, Six6 opposite strand transcript 1
计算分子量 587 aa, 68 kDa
观测分子量 50 kDa
GenBank蛋白编号BC125070
基因名称 C14orf39
Gene ID (NCBI) 317761
RRIDAB_2879146
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1H7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIX6OS1 antibody 22664-1-APDownload protocol
IHC protocol for SIX6OS1 antibody 22664-1-APDownload protocol
IF protocol for SIX6OS1 antibody 22664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Commun

C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility.

Authors - Laura Gómez-H
  • KO Validated
View Article