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验证数据展示

  • WB analysis of HeLa using 26130-1-AP

    WB analysis of HeLa using 26130-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26130-1-AP (C14orf28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 26130-1-AP

    IP experiment of HeLa using 26130-1-AP

    IP result of anti-C14orf28 (IP:26130-1-AP, 4ug; Detection:26130-1-AP 1:300) with HeLa cells lysate 1200ug.

  • IF Staining of HeLa using 26130-1-AP

    IF Staining of HeLa using 26130-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using C14orf28 antibody (26130-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C14orf28 Polyclonal antibody

C14orf28 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 26130-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

DRIP1, Dopamine receptor-interacting protein 1, c14_5270

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26130-1-AP targets C14orf28 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23441

Product name: Recombinant human C14orf28 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-310 aa of BC105030

Sequence: MKTLFEEIKASIKNNYNQDRSFCRPVLPWGGVFTIKAGRKAVSCTPLYVEIRLKNTCTIDGFLMLLYVILNENENFPRELSLHFGREFVDCFLYLMDTYSFTTVKLLWIWDKMEKQQYKSEVHKASLIIDLFGNEHDNFTKNLENLMSTIQESYCSNWRCPTRVQEDQQRTININPPQEIPHGNLIRLAVNELFCSKIELCEEHGCGGLREFSQRIFCHGAPPFVVLNMQHWKSEDLAYVPYYLDLSDHKYLLEGATLFNKEEHHYSAAFQIGGHWMHYDGLRNVNLILLNKPPEFLLLSSLVYIRATEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 28
别名 DRIP1, Dopamine receptor-interacting protein 1, c14_5270
观测分子量 35-40 kDa
GenBank蛋白编号BC105030
基因名称 C14orf28
Gene ID (NCBI) 122525
RRIDAB_2880393
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4W4Y0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C14orf28 antibody 26130-1-APDownload protocol
IF protocol for C14orf28 antibody 26130-1-APDownload protocol
IP protocol for C14orf28 antibody 26130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols