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C14orf2 Polyclonal antibody

C14orf2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14704-1-AP

Print datasheet

Synonyms

C14orf2, MP68, PLPM

验证数据展示

  • WB analysis of Jurkat using 14704-1-AP

    WB analysis of Jurkat using 14704-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14704-1-AP (C14orf2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 14704-1-AP

    IP experiment of Jurkat using 14704-1-AP

    IP result of anti-C14orf2 (IP:14704-1-AP, 4ug; Detection:14704-1-AP 1:300) with Jurkat cells lysate 3360ug.

  • IHC staining of human heart using 14704-1-AP

    IHC staining of human heart using 14704-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14704-1-AP (C14orf2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 14704-1-AP

    IHC staining of human heart using 14704-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14704-1-AP (C14orf2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, human brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14704-1-AP targets C14orf2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen C14orf2 fusion protein Ag6386 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 14 open reading frame 2
Synonyms C14orf2, MP68, PLPM
Calculated Molecular Weight 7 kDa
Observed Molecular Weight 7 kDa
GenBank Accession NumberBC001944
Gene Symbol C14orf2
Gene ID (NCBI) 9556
RRIDAB_2228192
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C14orf2 antibody 14704-1-APDownload protocol
IHC protocol for C14orf2 antibody 14704-1-APDownload protocol
IP protocol for C14orf2 antibody 14704-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols