- Featured Product
- KD/KO Validated
C14orf166 Polyclonal antibody
C14orf166 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse pancreas tissue, mouse lung tissue, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy RTRAF/C14orf166 Ready-To-Use IHC Kit. RTRAF/C14orf166 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 4 publications below |
产品信息
The immunogen of 19848-1-AP is C14orf166 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | C14orf166 fusion protein Ag13900 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chromosome 14 open reading frame 166 |
| Synonyms | C14orf166, CGI 99, CGI99, CLE, CLE7, LCRP369, RLLM1, RTRAF, UPF0568 protein C14orf166 |
| Calculated Molecular Weight | 244 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC001722 |
| Gene Symbol | C14orf166 |
| Gene ID (NCBI) | 51637 |
| RRID | AB_10646485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y224 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human CLE/C14orf166 protein is a positive modulator of cellular RNA polymerase. CLE is also shown to interact with the influenza virus polymerase complex and colocalizes with viral ribonucleoproteins, and is required for viral replication. Similarly, CLE is identified as a novel host interacting partners of the mature hepatitis C virus core protein. CLE levels were significantly higher in the serum of patients with PaCa compared with the control group was strongly expressed in tumor cells, suggesting that C14orf166 may be a potential biomarker of pancreatic adenocarcinoma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C14orf166 antibody 19848-1-AP | Download protocol |
| IHC protocol for C14orf166 antibody 19848-1-AP | Download protocol |
| IF protocol for C14orf166 antibody 19848-1-AP | Download protocol |
| IP protocol for C14orf166 antibody 19848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing. | ||
Biosci Rep Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small cell lung cancer. | ||
J Transl Med C14orf166 overexpression correlates with tumor progression and poor prognosis of breast cancer.
| ||
Tumour Biol C14ORF166 overexpression is associated with pelvic lymph node metastasis and poor prognosis in uterine cervical cancer. | ||
Tumour Biol Overexpression of chromosome 14 open reading frame 166 correlates with disease progression and poorer prognosis in human NPC. | ||
Mol Med Rep Expression and clinical significance of C14orf166 in esophageal squamous cell carcinoma. |
