验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse pancreas tissue, mouse lung tissue, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19848-1-AP targets C14orf166 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | C14orf166 fusion protein Ag13900 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 14 open reading frame 166 |
| 别名 | RTRAF, CLE7 homolog, CLE7, CLE, CGI-99 |
| 计算分子量 | 244 aa, 28 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC001722 |
| 基因名称 | C14orf166 |
| Gene ID (NCBI) | 51637 |
| RRID | AB_10646485 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y224 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human CLE/C14orf166 protein is a positive modulator of cellular RNA polymerase. CLE is also shown to interact with the influenza virus polymerase complex and colocalizes with viral ribonucleoproteins, and is required for viral replication. Similarly, CLE is identified as a novel host interacting partners of the mature hepatitis C virus core protein. CLE levels were significantly higher in the serum of patients with PaCa compared with the control group was strongly expressed in tumor cells, suggesting that C14orf166 may be a potential biomarker of pancreatic adenocarcinoma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C14orf166 antibody 19848-1-AP | Download protocol |
| IHC protocol for C14orf166 antibody 19848-1-AP | Download protocol |
| IF protocol for C14orf166 antibody 19848-1-AP | Download protocol |
| IP protocol for C14orf166 antibody 19848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing. | ||
Biosci Rep Overexpressed C14orf166 associates with disease progression and poor prognosis in non-small cell lung cancer. | ||
J Transl Med C14orf166 overexpression correlates with tumor progression and poor prognosis of breast cancer.
| ||
Tumour Biol C14ORF166 overexpression is associated with pelvic lymph node metastasis and poor prognosis in uterine cervical cancer. | ||
Tumour Biol Overexpression of chromosome 14 open reading frame 166 correlates with disease progression and poorer prognosis in human NPC. | ||
Mol Med Rep Expression and clinical significance of C14orf166 in esophageal squamous cell carcinoma. |
