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验证数据展示

  • WB analysis of PC-3 using 24722-1-AP (same clone as 24722-1-PBS)

    WB analysis of PC-3 using 24722-1-AP (same clone as 24722-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24722-1-AP (C14orf138 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 24722-1-AP (same clone as 24722-1-PBS)

    IHC staining of mouse testis using 24722-1-AP (same clone as 24722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24722-1-AP (C14orf138 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24722-1-PBS in a different storage buffer formulation.

C14orf138 Polyclonal antibody, PBS Only

C14orf138 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24722-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24722-1-PBS targets C14orf138 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20154

Product name: Recombinant human C14orf138 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-144 aa of BC105118

Sequence: MADTLESSLEDPLRSFVRVLEKRDGTVLRLQQYSSGGVGCVVWDAAIVLSKYLETPEFSGDGAHALSRRSVLELGSGTGAVGLMAATLGADVVVTDLEELQDLLKMNINMNKHLVTGSVQAKGGRNRRLSFSTRLHTDGRLHIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 14 open reading frame 138
别名
计算分子量 229 aa, 26 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC105118
基因名称 C14orf138
Gene ID (NCBI) 79609
RRIDAB_2879689
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H867
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C14orf138, also named as VCPKMT or METTL21D, is 229 amino acid protein, which belongs to the methyltransferase superfamily. C14orf138 localizes in the cytoplasm and interacts with ALKBH6. C14orf138 as a Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. C14orf138 exists as five isoforms. The calculated molecular weight of C14orf138 is 26 kDa,but we always detected a 46-50 kDa portein by western blot.

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