验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24968-1-AP targets NRDE2 in WB, IHC, IP, CoIP, chIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP, chIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag21805 Product name: Recombinant human C14orf102 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 615-933 aa of BC118673 Sequence: AVHILTKLTESSPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQYLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFHMKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKPLEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQCPLLWRMYLNFLVSLGNKERSKGVFYKALQSCPWAKVLYLDAVEYFPDEMQEILDLMTEKELRVRLPLEELELLLED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 14 open reading frame 102 |
| 别名 | C14orf102, Nuclear exosome regulator NRDE2, Protein NRDE2 homolog, UPF0614 protein C14orf102 |
| 计算分子量 | 1164 aa, 133 kDa |
| 观测分子量 | 133 kDa |
| GenBank蛋白编号 | BC118673 |
| 基因名称 | NRDE2 |
| Gene ID (NCBI) | 55051 |
| RRID | AB_2879825 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H7Z3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for NRDE2 antibody 24968-1-AP | Download protocol |
| WB protocol for NRDE2 antibody 24968-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression. | ||
RNA Biol NRDE-2, the human homolog of fission yeast Nrl1, prevents DNA damage accumulation in human cells. | ||
J Virol Kaposi's Sarcoma-Associated Herpesvirus Fine-Tunes the Temporal Expression of Late Genes by Manipulating a Host RNA Quality Control Pathway.
| ||
Cell Genom NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors
| ||
Genes Dev NRDE2 negatively regulates exosome functions by inhibiting MTR4 recruitment and exosome interaction.
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