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C13orf18 Polyclonal antibody

C13orf18 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21183-1-AP

Print datasheet

Synonyms

C13orf18

验证数据展示

  • WB analysis of human skeletal muscle using 21183-1-AP

    WB analysis of human skeletal muscle using 21183-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21183-1-AP (C13orf18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 21183-1-AP

    IHC staining of human testis using 21183-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 21183-1-AP

    IHC staining of human testis using 21183-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21183-1-AP (C13orf18 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 21183-1-AP

    IF Staining of HepG2 using 21183-1-AP

    Immunofluorescent analysis of HepG2 cells, using C13orf18 antibody 21183-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, MCF-7 cells
Positive IHC detected inhuman testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 21183-1-AP is C13orf18 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen C13orf18 fusion protein Ag15474 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 13 open reading frame 18
Synonyms C13orf18
Calculated Molecular Weight 662 aa, 73 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC032311
Gene Symbol C13orf18
Gene ID (NCBI) 80183
RRIDAB_10733098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H714
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C13orf18 antibody 21183-1-APDownload protocol
IHC protocol for C13orf18 antibody 21183-1-APDownload protocol
IF protocol for C13orf18 antibody 21183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Architecture of the human interactome defines protein communities and disease networks.

Authors - Edward L Huttlin
View Article
humanWB

Precis Clin Med

Blockade of Syk modulates neutrophil immune-responses via the mTOR/RUBCNL-dependent autophagy pathway to alleviate intestinal inflammation in ulcerative colitis

Authors - Fengqin Zhu
View Article