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验证数据展示

  • IF Staining of PC-12 using 26510-1-AP

    IF Staining of PC-12 using 26510-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using C13orf1 antibody (26510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • WB analysis of mouse brain using 26510-1-AP

    WB analysis of mouse brain using 26510-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26510-1-AP (C13orf1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 26510-1-AP

    IHC staining of human lymphoma using 26510-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26510-1-AP (C13orf1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 26510-1-AP

    IHC staining of human lymphoma using 26510-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 26510-1-AP (C13orf1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C13orf1 Polyclonal antibody

C13orf1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26510-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

SPRYD7, SPRY7, SPRY domain-containing protein 7, CLLD6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26510-1-AP targets C13orf1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C13orf1 fusion protein Ag23487 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 13 open reading frame 1
别名 SPRYD7, SPRY7, SPRY domain-containing protein 7, CLLD6
观测分子量 17 kDa, 20 kDa
GenBank蛋白编号BC022519
基因名称 C13orf1
Gene ID (NCBI) 57213
RRIDAB_2880536
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5W111
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C13orf1, also named as SPRYD7 and CLLD6, is involved in hematopoiesis via NOTCH signaling. C13orf1 plays an important role in the pathogenesis of B-CLL. There're some isoforms of C13orf1 with MW 15-17 kDa and 19-22 kDa.3

实验方案

Product Specific Protocols
WB protocol for C13orf1 antibody 26510-1-APDownload protocol
IHC protocol for C13orf1 antibody 26510-1-APDownload protocol
IF protocol for C13orf1 antibody 26510-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols