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验证数据展示

  • WB analysis using 20609-1-AP

    WB analysis using 20609-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20609-1-AP (C12orf51 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

C12orf51 Polyclonal antibody

C12orf51 Polyclonal Antibody for WB, ELISA
Cat No. 20609-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

HECTD4, Probable E3 ubiquitin-protein ligase HECTD4, KIAA0614, HECT-type E3 ubiquitin transferase HECTD4, HECT domain-containing protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20609-1-AP targets C12orf51 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 C12orf51 fusion protein Ag14614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 12 open reading frame 51
别名 HECTD4, Probable E3 ubiquitin-protein ligase HECTD4, KIAA0614, HECT-type E3 ubiquitin transferase HECTD4, HECT domain-containing protein 4
计算分子量 3996 aa, 439 kDa
观测分子量500 kDa
GenBank蛋白编号BC006270
基因名称 C12orf51
Gene ID (NCBI) 283450
RRIDAB_3669337
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HECT domain E3 ubiquitin protein ligase 4 (HECTD4), is also known as C12orf51. It may be involved in protein modification or ubiquitination (PMID: 26891264). In addition, genetic polymorphisms of C12orf51 are related to alcohol consumption and type 2 diabetes (T2D) (PMID: 38523829).

实验方案

Product Specific Protocols
WB protocol for C12orf51 antibody 20609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols