验证数据展示
经过测试的应用
| Positive IP detected in | HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29660-1-AP targets C12orf45 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | C12orf45 fusion protein Ag31258 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 12 open reading frame 45 |
| 别名 | NOPCHAP1 |
| 计算分子量 | 188 aa, 20 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC032326 |
| 基因名称 | C12orf45 |
| Gene ID (NCBI) | 121053 |
| RRID | AB_3669692 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N5I9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C12ORF45, also known as NOPCHAP1, is an assembly factor for the NOP-protein chaperone 1, which is an intrinsically disordered protein in free state. NOPCHAP1 plays a bridging role in the assembly of C/D snoRNPs, facilitating the selection of NOP58 and the assembly of box C/D snoRNPs.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for C12orf45 antibody 29660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |