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验证数据展示

  • WB analysis using 33680-1-AP

    WB analysis using 33680-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33680-1-AP (C12orf35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 33680-1-AP

    WB analysis of K-562 using 33680-1-AP

    WB result of C12orf35 antibody (33680-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C12orf35 transfected K-562 cells.

  • Featured Product
  • KD/KO Validated

RESF1 Polyclonal antibody

RESF1 Polyclonal Antibody for WB, ELISA
Cat No. 33680-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C12orf35, KIAA1551, Retroelement silencing factor 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCaco-2 cells, K-562 cells, HUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33680-1-AP targets RESF1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38168

Product name: Recombinant human C12orf35 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1399-1747 aa of BC140819

Sequence: GSVGATFKLGDSLSNPNERAIVKEKMVSNTKSVDTKASSSKFSRILTPKEYLQRQKHKEALSNKASKKICVKNVPCDSEHMRPSKLAVQVESCGKSNEKHSSGVQTSKESLNGLTSHGKNLKIHHSQESKTYNILRNVKEKVGGKQPDKIWIDKTKLDKLTNISNEAQFSQMPPQVKDQKKLYLNRVGFKCTERESISLTKLESSPRKLHKDKRQENKHKTFLPVKGNTEKSNMLEFKLCPDILLKNTNSVEERKDVKPHPRKEQAPLQVSGIKSTKEDWLKFVATKKRTQKDSQERDNVNSRLSKRSFSADGFEMLQNPVKDSKEMFQTYKQMYLEKRSRSLGSSPVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 12 open reading frame 35
别名 C12orf35, KIAA1551, Retroelement silencing factor 1
计算分子量 1747 aa, 195 kDa
观测分子量240-250 kDa
GenBank蛋白编号BC140819
基因名称 C12orf35
Gene ID (NCBI) 55196
RRIDAB_3743047
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9HCM1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RESF1 (Retroelement Silencing Factor 1, also known as C12orf35 or KIAA1551) is a nuclear protein that primarily mediates the epigenetic silencing of endogenous retroviruses (ERVs) and imprinted genes by interacting with the SETDB1 histone methyltransferase, thereby maintaining H3K9me3 modifications and heterochromatin conformation. Recent studies have revealed that RESF1 exerts metastasis-suppressive functions in estrogen receptor-negative breast cancer. Additionally, RESF1 negatively regulates ribosomal biogenesis and global protein translation, serving as a multifunctional factor that links genome stability, stem cell pluripotency, and tumor suppression.

实验方案

Product Specific Protocols
WB protocol for RESF1 antibody 33680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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