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验证数据展示

  • WB analysis of HeLa using 31748-1-AP

    WB analysis of HeLa using 31748-1-AP

    WB result of C12orf30 antibody (31748-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C12orf30 transfected HeLa cells.

  • WB analysis using 31748-1-AP

    WB analysis using 31748-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31748-1-AP (C12orf30 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse ovary using 31748-1-AP

    IP experiment of mouse ovary using 31748-1-AP

    IP result of anti-C12orf30 (IP:31748-1-AP, 4ug; Detection:31748-1-AP 1:1500) with mouse ovary tissue lysate 640 ug.

  • IP experiment of HeLa using 31748-1-AP

    IP experiment of HeLa using 31748-1-AP

    IP result of anti-C12orf30 (IP:31748-1-AP, 4ug; Detection:31748-1-AP 1:2000) with HeLa cells lysate 1280 ug.

  • IHC staining of human cervical cancer using 31748-1-AP

    IHC staining of human cervical cancer using 31748-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 31748-1-AP (C12orf30 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 31748-1-AP

    IHC staining of human colon cancer using 31748-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 31748-1-AP (C12orf30 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 31748-1-AP

    IF Staining of A431 using 31748-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using C12orf30 antibody (31748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

C12orf30 Polyclonal antibody

C12orf30 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 31748-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

MDM20, NAA25, N-alpha-acetyltransferase 25, NatB auxiliary subunit, NAP1, N-terminal acetyltransferase B complex subunit MDM20

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Calu-1 cells
Positive IP detected inmouse ovary tissue, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31748-1-AP targets C12orf30 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36383

Product name: Recombinant human C12orf30 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 703-972 aa of BC113585

Sequence: LNHPVEPKNSEKTAENGVSSRIDILRLLLQQLEATLETGKRFIEKDIQYPFLGPVPTRMGGFFNSGCSQCQISSFYLVNDIYELDTSGLEDTMEIQERIENSFKSLLDQLKDVFSKCKGDLLEVKDGNLKTHPTLLENLVFFVETISVILWVSSYCESVLRPYKLNLQKKKKKKKETSIIMPPVFTSFQDYVTGLQTLISNVVDHIKGLETHLIALKLEELILEDTSLSPEERKFSKTVQGKVQSSYLHSLLEMGELLKKRLETTKKLKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 12 open reading frame 30
别名 MDM20, NAA25, N-alpha-acetyltransferase 25, NatB auxiliary subunit, NAP1, N-terminal acetyltransferase B complex subunit MDM20
观测分子量113 kDa，140 kDa
GenBank蛋白编号BC113585
基因名称 C12orf30
Gene ID (NCBI) 80018
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ14CX7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The C12orf30 gene encodes an auxiliary subunit of the N-terminal acetyltransferase B (NatB) complex, which is responsible for the acetylation of methionine residues at the N-terminus of proteins. The C12orf30 protein plays a role in the distribution and morphology of organelles, and is involved in cell migration and the dynamic changes of the cytoskeleton. In addition, polymorphisms in the C12orf30 gene are associated with susceptibility to various autoimmune diseases, such as rheumatoid arthritis, juvenile idiopathic arthritis, and type 1 diabetes. The C12orf30 protein also participates in regulating the function of immune cells, influencing their signal transduction. The theoretical size of C12orf30 protein is 113 kDa, and it is possible that post-translational modifications may have shifted the protein band up to about 140 kD.

实验方案

Product Specific Protocols
IF protocol for C12orf30 antibody 31748-1-APDownload protocol
IHC protocol for C12orf30 antibody 31748-1-APDownload protocol
IP protocol for C12orf30 antibody 31748-1-APDownload protocol
WB protocol for C12orf30 antibody 31748-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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