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验证数据展示

  • WB analysis of HeLa using 84708-1-RR

    WB analysis of HeLa using 84708-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84708-1-RR (C12orf29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84708-1-RR

    Affinity and Kinetic Characterization of 84708-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84708-1-RR against Human C12orf29 were performed. The affinity constant is below 1 pM.

C12orf29 Recombinant antibody

C12orf29 Recombinant Antibody for WB, ELISA
Cat No. 84708-1-RR

产品说明书

CloneNo. 242163C3

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

Rnl, RNA Ligase 1, RNA Ligase, 242163C3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U-87 MG cells, LNCaP cells, K-562 cells, rat pancreas tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84708-1-RR targets C12orf29 in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 C12orf29 fusion protein Ag35681 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 12 open reading frame 29
别名 Rnl, RNA Ligase 1, RNA Ligase, 242163C3
计算分子量37kDa，325aa
观测分子量37 kDa
GenBank蛋白编号NM_001009894.2
基因名称 C12orf29
Gene ID (NCBI) 91298
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N999
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).

实验方案

Product Specific Protocols
WB protocol for C12orf29 antibody 84708-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols