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验证数据展示

  • WB analysis using 27754-1-AP

    WB analysis using 27754-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27754-1-AP (C11orf82 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

C11orf82 Polyclonal antibody

C11orf82 Polyclonal Antibody for WB, ELISA
Cat No. 27754-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

noxin, Nitric oxide-inducible gene protein, DNA damage-induced apoptosis suppressor protein, DNA Damage Induced Apoptosis Suppressor, DDIAS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, NCI-H1299 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27754-1-AP targets C11orf82 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 C11orf82 fusion protein Ag26829 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 82
别名 noxin, Nitric oxide-inducible gene protein, DNA damage-induced apoptosis suppressor protein, DNA Damage Induced Apoptosis Suppressor, DDIAS
观测分子量120 kDa
GenBank蛋白编号BC039268
基因名称 C11orf82
Gene ID (NCBI) 220042
RRIDAB_3669620
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IXT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DNA damage-induced apoptosis suppressor (DDIAS) is an oncogenic protein that is highly expressed in several cancers, including colorectal, lung, breast, and hepatocellular carcinoma (HCC), and stimulates cancer cell proliferation and cell cycle progression.DDIAS protects cancer cells from DNA damage reagents and tumor necrosis factor-associated apoptosis-inducing ligands (TRAILs) and positively regulates cancer cell invasion by stabilizing the β- catenin positively regulates cancer cell invasion. Therefore, DDIAS is considered as a potential therapeutic target for malignant lung cancer.

实验方案

Product Specific Protocols
WB protocol for C11orf82 antibody 27754-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols