验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IHC detected in | human testis tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19906-1-AP targets SDH5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag13893 Product name: Recombinant human C11orf79 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC002331 Sequence: MAVSTVFSTSSLMLALSRHSLLSPLLSVTSFRRFYRGDSPTDSQKDMIEIPLPPWQERTDESIETKRARLLYESRKRGMLENCILLSLFAKEHLQHMTEKQLNLYDRLINEPSNDWDIYYWATEAKPAPEIFENEVMALLRDFAKNKNKEQRLRAPDLEYLFEKPR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 11 open reading frame 79 |
| 别名 | C11orf79, hSDH5, PGL2, SDH assembly factor 2, SDH5, SDHAF2, SDHAF2, PGL2, SDH5 |
| 计算分子量 | 166 aa, 20 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC002331 |
| 基因名称 | SDH5 |
| Gene ID (NCBI) | 54949 |
| RRID | AB_2878621 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NX18 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDH5, also named asC11orf79, SDHAF2 and SDHAF2, is a mitochondrial protein needed for the flavination of a succinate dehydrogenase complex subunit required for activity of the complex. SDH5 has been shown to be mutated in patients with paragangliomas, also known as familial non-chromaffin paragangliomas type 2. Catalog#19906-1-AP is a rabbit polyclonal antibody raised against the full-length of human SDH5.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SDH5 antibody 19906-1-AP | Download protocol |
| IHC protocol for SDH5 antibody 19906-1-AP | Download protocol |
| IF protocol for SDH5 antibody 19906-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. | ||
Apoptosis GAS6/Axl is associated with AMPK activation and attenuates H2O2-induced oxidative stress | ||
Cell Biochem Funct Pterostilbene protects against H2 O2 -induced oxidative stress by regulating GAS6/Axl signaling in HL-1 cells | ||
Biochim Biophys Acta Mol Basis Dis Succinate dehydrogenase mediated ROS production contributes to ASIC1a-induced chondrocyte pyroptosis in rheumatoid arthritis |
