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验证数据展示

  • WB analysis of mouse skeletal muscle using 25758-1-AP

    WB analysis of mouse skeletal muscle using 25758-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25758-1-AP (C11orf74 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 25758-1-AP

    IHC staining of human liver cancer using 25758-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25758-1-AP (C11orf74 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 25758-1-AP

    IHC staining of human liver cancer using 25758-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25758-1-AP (C11orf74 Antibody) at dilution of 1:50 (under 40x lens).

C11orf74 Polyclonal antibody

C11orf74 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25758-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C11orf74, Protein HEPIS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25758-1-AP targets C11orf74 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag22770

Product name: Recombinant human C11orf74 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-221 aa of BC051031

Sequence: MSAHMSGLEIMDEDQLIKDVLDKFLNCHEQTYDEEFLNTFTHLSQEDHVSKRGVFGTDSSENIFTSAKVTHKNEADDYHLRNKTIFLRTSSQCLEEQVDNFLDLEDLDMDEEIKPQMSEDLLLLPGEVEQDVSTSIPSCIPFVAQPPTCEVKPKPSVKRMDKQTEEILGDEVQLFSLDEEFDYDNVMLTSKFSPAEIENIKELCKQQKRKDTSPDLEKSCD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 74
别名 C11orf74, Protein HEPIS
计算分子量 221 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC051031
基因名称 C11orf74
Gene ID (NCBI) 119710
RRIDAB_2880227
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VG3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C11orf74, also named as Protein HEPIS, has two isoforms with MW 25 kDa and 17 kDa.

实验方案

Product Specific Protocols
WB protocol for C11orf74 antibody 25758-1-APDownload protocol
IHC protocol for C11orf74 antibody 25758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols