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验证数据展示

  • WB analysis of mouse testis using 20780-1-AP

    WB analysis of mouse testis using 20780-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20780-1-AP (C11orf70 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20780-1-AP

    IHC staining of human testis using 20780-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20780-1-AP (C11orf70 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 20780-1-AP

    IHC staining of human testis using 20780-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20780-1-AP (C11orf70 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 20780-1-AP

    IF Staining of HeLa using 20780-1-AP

    Immunofluorescent analysis of Hela cells, using C11orf70 antibody 20780-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

C11orf70 Polyclonal antibody

C11orf70 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20780-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

CK070, Cilia- and flagella-associated protein 300, CFAP300

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, MCF-7 cells
Positive IHC detected inhuman testis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20780-1-AP targets C11orf70 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 C11orf70 fusion protein Ag14793 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 70
别名 CK070, Cilia- and flagella-associated protein 300, CFAP300
计算分子量 267 aa, 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC006128
基因名称 C11orf70
Gene ID (NCBI) 85016
RRIDAB_10755147
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRQ4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C11orf70 antibody 20780-1-APDownload protocol
IHC protocol for C11orf70 antibody 20780-1-APDownload protocol
IF protocol for C11orf70 antibody 20780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols