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验证数据展示

  • IHC staining of human ovary tumor using 20414-1-AP

    IHC staining of human ovary tumor using 20414-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20414-1-AP (C11orf67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 20414-1-AP

    WB analysis of mouse testis using 20414-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20414-1-AP (C11orf67 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 20414-1-AP

    IHC staining of human ovary tumor using 20414-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20414-1-AP (C11orf67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 20414-1-AP

    WB analysis of HepG2 using 20414-1-AP

    WB result of C11orf67 antibody (20414-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-C11orf67 transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

C11orf67 Polyclonal antibody

C11orf67 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20414-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AAMDC, Adipogenesis associated Mth938 domain-containing protein, CK067, Mth938 domain-containing protein, PTD015

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, HepG2 cells, rat testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20414-1-AP targets C11orf67 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 C11orf67 fusion protein Ag14258 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 67
别名 AAMDC, Adipogenesis associated Mth938 domain-containing protein, CK067, Mth938 domain-containing protein, PTD015
计算分子量 122 aa, 13 kDa
GenBank蛋白编号BC002752
基因名称 C11orf67
Gene ID (NCBI) 28971
RRIDAB_10694438
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7C9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C11orf67 is predicted to be involved in positive regulation of fat cell differentiation.

实验方案

Product Specific Protocols
WB protocol for C11orf67 antibody 20414-1-APDownload protocol
IHC protocol for C11orf67 antibody 20414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols