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验证数据展示

  • IHC staining of human ovary tumor using 20414-1-AP

    IHC staining of human ovary tumor using 20414-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20414-1-AP (C11orf67 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 20414-1-AP

    WB analysis of mouse testis using 20414-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20414-1-AP (C11orf67 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 20414-1-AP

    IHC staining of human ovary tumor using 20414-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20414-1-AP (C11orf67 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 20414-1-AP

    WB analysis of HepG2 using 20414-1-AP

    WB result of C11orf67 antibody (20414-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-C11orf67 transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

C11orf67 Polyclonal antibody

C11orf67 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20414-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AAMDC, Adipogenesis associated Mth938 domain-containing protein, CK067, Mth938 domain-containing protein, PTD015

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, HepG2 cells, rat testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20414-1-AP targets C11orf67 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14258

Product name: Recombinant human C11orf67 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-122 aa of BC002752

Sequence: MTSPEIASLSWGQMKVKGSNTTYKDCKVWPGGSRTWDWRETGTEHSPGVQPADVKEVVEKGVQTLVIGRGMSEALKVPSSTVEYLKKHGIDVRVLQTEQAVKEYNALVAQGVRVGGVFHSTC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 67
别名 AAMDC, Adipogenesis associated Mth938 domain-containing protein, CK067, Mth938 domain-containing protein, PTD015
计算分子量 122 aa, 13 kDa
观测分子量13 kDa
GenBank蛋白编号BC002752
基因名称 C11orf67
Gene ID (NCBI) 28971
RRIDAB_10694438
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7C9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C11orf67 is predicted to be involved in positive regulation of fat cell differentiation.

实验方案

Product Specific Protocols
WB protocol for C11orf67 antibody 20414-1-APDownload protocol
IHC protocol for C11orf67 antibody 20414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols