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验证数据展示

  • IHC staining of human breast cancer using 21181-1-AP

    IHC staining of human breast cancer using 21181-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21181-1-AP (C11orf57 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 21181-1-AP

    IHC staining of human breast cancer using 21181-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21181-1-AP (C11orf57 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse brain using 21181-1-AP

    WB analysis of mouse brain using 21181-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21181-1-AP (C11orf57 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

C11orf57 Polyclonal antibody

C11orf57 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21181-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

C11orf57

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21181-1-AP targets C11orf57 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 C11orf57 fusion protein Ag15471 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 57
别名 C11orf57
计算分子量 293 aa, 34 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC030546
基因名称 C11orf57
Gene ID (NCBI) 55216
RRIDAB_2878825
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZUT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C11orf57 has three isoforms with MW 30-34 kDa.

实验方案

Product Specific Protocols
WB protocol for C11orf57 antibody 21181-1-APDownload protocol
IHC protocol for C11orf57 antibody 21181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways

Authors - Min Huang
View Article