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验证数据展示

  • WB analysis of HT-29 using 20409-1-AP

    WB analysis of HT-29 using 20409-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20409-1-AP

    IHC staining of human placenta using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of human lung using 20409-1-AP

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of human lung using 20409-1-AP

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of mouse adrenal gland using 20409-1-AP

    IHC staining of mouse adrenal gland using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of mouse lung using 20409-1-AP

    IHC staining of mouse lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of mouse spleen using 20409-1-AP

    IHC staining of mouse spleen using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of rat adrenal gland using 20409-1-AP

    IHC staining of rat adrenal gland using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included
  • IHC staining of rat lung using 20409-1-AP

    IHC staining of rat lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 20409-1-AP (APC15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANAPC15 antibody included

APC15 Polyclonal antibody

APC15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20409-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C11orf51, HSPC020, Anaphase-promoting complex subunit 15, ANAPC15

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, HEK-293 cells
Positive IHC detected inhuman placenta tissue, human lung tissue, human pancreas tissue, mouse adrenal gland tissue, mouse lung tissue, mouse spleen tissue, rat adrenal gland tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ANAPC15 Ready-To-Use IHC Kit. ANAPC15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20409-1-AP targets APC15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14237

Product name: Recombinant human C11orf51 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-121 aa of BC005156

Sequence: MSTLFPSLFPRVTETLWFNLDRPCVEETELQQQEQQHQAWLQSIAEKDNNLVPIGKPASEHYDDEEEEDDEDDEDSEEDSEDDEDMQDMDEMNDYNESPDDGEVNEVDMEGNEQDQDQWMI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 51
别名 C11orf51, HSPC020, Anaphase-promoting complex subunit 15, ANAPC15
计算分子量 121 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC005156
基因名称 APC15
Gene ID (NCBI) 25906
RRIDAB_10694279
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP60006
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for APC15 antibody 20409-1-APDownload protocol
WB protocol for APC15 antibody 20409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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