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APC15 Polyclonal antibody

APC15 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20409-1-AP

Print datasheet

Synonyms

APC15, C11orf51, HSPC020

验证数据展示

  • WB analysis of HT-29 using 20409-1-AP

    WB analysis of HT-29 using 20409-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 20409-1-AP (APC15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 20409-1-AP

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 20409-1-AP

    IHC staining of human lung using 20409-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20409-1-AP (C11orf51 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHT-29 cells, HEK-293 cells
Positive IHC detected inhuman lung tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20409-1-AP is APC15 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen APC15 fusion protein Ag14237 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 11 open reading frame 51
Synonyms APC15, C11orf51, HSPC020
Calculated Molecular Weight 121 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC005156
Gene Symbol C11orf51
Gene ID (NCBI) 25906
RRIDAB_10694279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60006
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for APC15 antibody 20409-1-APDownload protocol
IHC protocol for APC15 antibody 20409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols