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验证数据展示

  • WB analysis of mouse lung using 20195-1-AP

    WB analysis of mouse lung using 20195-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20195-1-AP (C11orf49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 20195-1-AP

    IHC staining of human colon using 20195-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20195-1-AP (C11orf49 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon using 20195-1-AP

    IHC staining of human colon using 20195-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 20195-1-AP (C11orf49 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20195-1-AP

    IF Staining of HepG2 using 20195-1-AP

    Immunofluorescent analysis of HepG2 cells, using C11orf49 antibody 20195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

C11orf49 Polyclonal antibody

C11orf49 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20195-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

C11orf49, UPF0705 protein C11orf49

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, human brain tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20195-1-AP targets C11orf49 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 C11orf49 fusion protein Ag14123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 49
别名 C11orf49, UPF0705 protein C11orf49
计算分子量 331 aa, 37 kDa
观测分子量 31 kDa
GenBank蛋白编号BC001860
基因名称 C11orf49
Gene ID (NCBI) 79096
RRIDAB_10646431
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6J7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C11orf49 antibody 20195-1-APDownload protocol
IHC protocol for C11orf49 antibody 20195-1-APDownload protocol
IF protocol for C11orf49 antibody 20195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Cell

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.

Authors - Edward L Huttlin
View Article