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验证数据展示

  • WB analysis of MCF-7 using 20919-1-AP

    WB analysis of MCF-7 using 20919-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20919-1-AP (C11orf42 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 20919-1-AP

    IF Staining of MCF-7 using 20919-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20919-1-AP (C11orf42 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 20919-1-AP

    IF Staining of MCF-7 using 20919-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using C11orf42 antibody (20919-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C11orf42 Polyclonal antibody

C11orf42 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20919-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

chromosome 11 open reading frame 42

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20919-1-AP targets C11orf42 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15118

Product name: Recombinant human C11orf42 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-333 aa of BC031612

Sequence: MLVGTPNLLTLDEADATWTLIKDKVIEEHFGPNAVAVPFLSDAACYDLLGVLVKQSRPAHTRLALPGRQGRRALKPVGPLPSLLEQAGSEGAFAHCTREYSPNGRAERAYEETRMLDGQPCKIRLHMGDLRKKVAFLLLPPGQVSLQQTLPWLRSTHSIYVIYQVFSCSWLQLGLTSTAREPQLLRLLRSLPVAFSCLKFSLQSKGVLGPQKPLTKDPLPHGANWVRPNLSIMPPLAPTSASADTTEAADVPPPVPAPPTPPPQEGPEDKPTRFSYKGRNPFWRGPQILSENWLFSPRSPPPGAQGGGPRDPDGHSMSLPLLQGLSSEFDSDD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 42
别名 chromosome 11 open reading frame 42
计算分子量 333 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC031612
基因名称 C11orf42
Gene ID (NCBI) 160298
RRIDAB_2878764
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ8N5U0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C11orf42 antibody 20919-1-APDownload protocol
IF protocol for C11orf42 antibody 20919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols