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验证数据展示

  • IHC staining of human skin using 20446-1-AP

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin using 20446-1-AP

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of ROS1728 using 20446-1-AP

    WB analysis of ROS1728 using 20446-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 20446-1-AP (C10orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of ROS1728 using 20446-1-AP

    IF Staining of ROS1728 using 20446-1-AP

    Immunofluorescent analysis of ROS1728 cells using 20446-1-AP (C10orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

C10orf58 Polyclonal antibody

C10orf58 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20446-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

C10orf58, F213A, FAM213A, PSEC0139, UNQ611/PRO1198, UPF0765 protein C10orf58

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inROS1728 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inROS1728 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

20446-1-AP targets C10orf58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, rat
文献引用反应性human
免疫原 C10orf58 fusion protein Ag14272 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 58
别名 C10orf58, F213A, FAM213A, PSEC0139, UNQ611/PRO1198, UPF0765 protein C10orf58
计算分子量 229 aa, 26 kDa
观测分子量 24-30 kDa
GenBank蛋白编号BC005871
基因名称 C10orf58
Gene ID (NCBI) 84293
RRIDAB_2878687
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C10orf58, also name as FAM213A and PAMM, is involved in redox regulation of the cell. It acts as an antioxidant. C10orf58 inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. It may affect bone resorption and help to maintain bone mass. The MW of C10orf58 is 24-30 kDa.

实验方案

Product Specific Protocols
WB protocol for C10orf58 antibody 20446-1-APDownload protocol
IHC protocol for C10orf58 antibody 20446-1-APDownload protocol
IF protocol for C10orf58 antibody 20446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
HumanIHC

Oncol Rep

Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1

Authors - Jie Zhang
View Article