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验证数据展示

  • IHC staining of human skin using 20446-1-AP

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin using 20446-1-AP

    IHC staining of human skin using 20446-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 20446-1-AP (C10orf58 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of ROS1728 using 20446-1-AP

    WB analysis of ROS1728 using 20446-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 20446-1-AP (C10orf58 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of ROS1728 using 20446-1-AP

    IF Staining of ROS1728 using 20446-1-AP

    Immunofluorescent analysis of ROS1728 cells using 20446-1-AP (C10orf58 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

C10orf58 Polyclonal antibody

C10orf58 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20446-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

C10orf58, F213A, FAM213A, PSEC0139, UNQ611/PRO1198, UPF0765 protein C10orf58

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inROS1728 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inROS1728 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20446-1-AP targets C10orf58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 C10orf58 fusion protein Ag14272 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 58
别名 C10orf58, F213A, FAM213A, PSEC0139, UNQ611/PRO1198, UPF0765 protein C10orf58
计算分子量 229 aa, 26 kDa
观测分子量 24-30 kDa
GenBank蛋白编号BC005871
基因名称 C10orf58
Gene ID (NCBI) 84293
RRIDAB_2878687
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C10orf58, also name as FAM213A and PAMM, is involved in redox regulation of the cell. It acts as an antioxidant. C10orf58 inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. It may affect bone resorption and help to maintain bone mass. The MW of C10orf58 is 24-30 kDa.

实验方案

Product Specific Protocols
WB protocol for C10orf58 antibody 20446-1-APDownload protocol
IHC protocol for C10orf58 antibody 20446-1-APDownload protocol
IF protocol for C10orf58 antibody 20446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols