验证数据展示
经过测试的应用
| Positive WB detected in | ROS1728 cells |
| Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | ROS1728 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
20446-1-AP targets C10orf58 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag14272 Product name: Recombinant human C10orf58 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-229 aa of BC005871 Sequence: DVFLSKPQKAALEYLEDIDLKTLEKEPRTFKAKELWEKNGAVIMAVRRPGCFLCREEAADLSSLKSMLDQLGVPLYAVVKEHIRTEVKDFQPYFKGEIFLDEKKKFYGPQRRKMMFMGFIRLGVWYNFFRAWNGGFSGNLEGEGFILGGVFVVGSGKQGILLEHREKEFGDKVNLLSVLEAAKMIKPQTLASEKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 10 open reading frame 58 |
| 别名 | F213A, FAM213A, PAMM, Peroxiredoxin-like 2 activated in M-CSF stimulated monocytes, Peroxiredoxin-like 2A |
| 计算分子量 | 229 aa, 26 kDa |
| 观测分子量 | 24-30 kDa |
| GenBank蛋白编号 | BC005871 |
| 基因名称 | C10orf58 |
| Gene ID (NCBI) | 84293 |
| RRID | AB_2878687 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRX8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C10orf58, also name as FAM213A and PAMM, is involved in redox regulation of the cell. It acts as an antioxidant. C10orf58 inhibits TNFSF11-induced NFKB1 and JUN activation and osteoclast differentiation. It may affect bone resorption and help to maintain bone mass. The MW of C10orf58 is 24-30 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for C10orf58 antibody 20446-1-AP | Download protocol |
| IHC protocol for C10orf58 antibody 20446-1-AP | Download protocol |
| WB protocol for C10orf58 antibody 20446-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
