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验证数据展示

  • WB analysis of A549 using 24967-1-AP

    WB analysis of A549 using 24967-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24967-1-AP (C10orf28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24967-1-AP

    IF Staining of HepG2 using 24967-1-AP

    Immunofluorescent analysis of HepG2 cells using 24967-1-AP (C10orf28 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human small intestine using 24967-1-AP

    IHC staining of human small intestine using 24967-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24967-1-AP (C10orf28 antibody) at dilution of 1:200 (under 40x lens).

C10orf28 Polyclonal antibody

C10orf28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24967-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Putative mitochondrial space protein 32.1, PSORT, Growth inhibition and differentiation-related protein 88, GIDRP88, GIDRP86

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, A431 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24967-1-AP targets C10orf28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 C10orf28 fusion protein Ag21740 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 28
别名 Putative mitochondrial space protein 32.1, PSORT, Growth inhibition and differentiation-related protein 88, GIDRP88, GIDRP86
计算分子量 792 aa, 88 kDa
观测分子量 110 kDa
GenBank蛋白编号BC098359
基因名称 C10orf28
Gene ID (NCBI) 27291
RRIDAB_2879824
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z5L2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C10orf28 antibody 24967-1-APDownload protocol
IHC protocol for C10orf28 antibody 24967-1-APDownload protocol
IF protocol for C10orf28 antibody 24967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols