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C10orf28 Polyclonal antibody

C10orf28 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24967-1-AP

Print datasheet

Synonyms

C10orf28, C10orf28 protein, GIDRP86, PSORT

验证数据展示

  • WB analysis of A549 using 24967-1-AP

    WB analysis of A549 using 24967-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 24967-1-AP (C10orf28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 24967-1-AP

    IHC staining of human small intestine using 24967-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24967-1-AP (C10orf28 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 24967-1-AP

    IF Staining of HepG2 using 24967-1-AP

    Immunofluorescent analysis of HepG2 cells using 24967-1-AP (C10orf28 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, A431 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24967-1-AP is C10orf28 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human
Immunogen C10orf28 fusion protein Ag21740 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 28
Synonyms C10orf28, C10orf28 protein, GIDRP86, PSORT
Calculated Molecular Weight 792 aa, 88 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC098359
Gene Symbol C10orf28
Gene ID (NCBI) 27291
RRIDAB_2879824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z5L2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C10orf28 antibody 24967-1-APDownload protocol
IHC protocol for C10orf28 antibody 24967-1-APDownload protocol
IF protocol for C10orf28 antibody 24967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols