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C10orf27 Polyclonal antibody

C10orf27 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24424-1-AP

Print datasheet

Synonyms

C10orf27, Protein SPATIAL, spatial, TBATA

验证数据展示

  • WB analysis of HeLa using 24424-1-AP

    WB analysis of HeLa using 24424-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24424-1-AP (C10orf27 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 24424-1-AP

    IHC staining of mouse brain using 24424-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24424-1-AP (C10orf27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24424-1-AP is C10orf27 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen C10orf27 fusion protein Ag19625 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 27
Synonyms C10orf27, Protein SPATIAL, spatial, TBATA
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC137543
Gene Symbol C10orf27
Gene ID (NCBI) 219793
RRIDAB_2879542
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96M53
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C10orf27, also named as TBATA and SPATIAL, may play a role in spermatid differentiation. We always got 40-45 kDa band in our detection.

实验方案

Product Specific Protocols
WB protocol for C10orf27 antibody 24424-1-APDownload protocol
IHC protocol for C10orf27 antibody 24424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols