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EDRF1 Polyclonal antibody

EDRF1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21883-1-AP

Print datasheet

Synonyms

C10orf137, EDRF1, RP11 383C5.6

验证数据展示

  • WB analysis using 21883-1-AP

    WB analysis using 21883-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 21883-1-AP

    IHC staining of human testis using 21883-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 21883-1-AP

    IHC staining of human testis using 21883-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21883-1-AP (EDRF1 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, PC-3 cells, HEK-293 cells, mouse brain tissue, MCF-7 cells
Positive IHC detected inhuman testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21883-1-AP is EDRF1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen EDRF1 fusion protein Ag16441 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 137
Synonyms C10orf137, EDRF1, RP11 383C5.6
Calculated Molecular Weight 1238 aa, 139 kDa
Observed Molecular Weight 140-160 kDa
GenBank Accession NumberBC107479
Gene Symbol C10orf137
Gene ID (NCBI) 26098
RRIDAB_11042452
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3B7T1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EDRF1, also named as C10orf137, is a transcription factor involved in erythroid differentiation. It regulates alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor. EDRF1 has four isoforms with MW 66 kDa, 89 kDa and 135-140 kDa.

实验方案

Product Specific Protocols
WB protocol for EDRF1 antibody 21883-1-APDownload protocol
IHC protocol for EDRF1 antibody 21883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols