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验证数据展示

  • WB analysis using 21883-1-AP

    WB analysis using 21883-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21883-1-AP (EDRF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 21883-1-AP

    IHC staining of human cervical cancer using 21883-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 21883-1-AP

    IHC staining of human cervical cancer using 21883-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21883-1-AP (EDRF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EDRF1 Polyclonal antibody

EDRF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21883-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

C10orf137, RP11 383C5.6, Erythroid differentiation-related factor 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, PC-3 cells, HEK-293 cells, mouse brain tissue, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21883-1-AP targets EDRF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 EDRF1 fusion protein Ag16441 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 137
别名 C10orf137, RP11 383C5.6, Erythroid differentiation-related factor 1
计算分子量 1238 aa, 139 kDa
观测分子量 140-160 kDa
GenBank蛋白编号BC107479
基因名称 EDRF1
Gene ID (NCBI) 26098
RRIDAB_11042452
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3B7T1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EDRF1, also named as C10orf137, is a transcription factor involved in erythroid differentiation. It regulates alpha- and gamma-globin gene synthesis by modulating DNA-binding activity of GATA-1 transcription factor. EDRF1 has four isoforms with MW 66 kDa, 89 kDa and 135-140 kDa.

实验方案

Product Specific Protocols
WB protocol for EDRF1 antibody 21883-1-APDownload protocol
IHC protocol for EDRF1 antibody 21883-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Differential localization of dengue virus protease affects cell homeostasis and triggers to thrombocytopenia

Authors - Lekha Gandhi
View Article