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验证数据展示

  • IP experiment of HEK-293 using 26324-1-AP

    IP experiment of HEK-293 using 26324-1-AP

    IP result of anti-C10orf104 (IP:26324-1-AP, 4ug; Detection:26324-1-AP 1:800) with HEK-293 cells lysate 1440 ug.

  • IHC staining of human stomach cancer using 26324-1-AP

    IHC staining of human stomach cancer using 26324-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26324-1-AP (C10orf104 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 26324-1-AP

    IF Staining of A431 using 26324-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using C10orf104 antibody (26324-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

C10orf104 Polyclonal antibody

C10orf104 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 26324-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IP, ELISA

别名

ANAPC16, Anaphase-promoting complex subunit 16, APC16, CENP-27, Cyclosome subunit 16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26324-1-AP targets C10orf104 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 C10orf104 fusion protein Ag23641 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 104
别名 ANAPC16, Anaphase-promoting complex subunit 16, APC16, CENP-27, Cyclosome subunit 16
观测分子量12 kDa
GenBank蛋白编号BC009530
基因名称 C10orf104
Gene ID (NCBI) 119504
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DE5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C10orf104 (also known as ANAPC16 or APC16) is a protein associated with the anaphase-promoting complex/cyclosome (APC/C). APC/C is a highly conserved E3 ubiquitin ligase that regulates various stages of the cell cycle, particularly the transition from mitotic to non-mitotic phases and the entry into the G1 phase during mitosis. As a subunit of APC/C, C10orf104 contributes to its stability and modulates its ubiquitin ligase activity.

实验方案

Product Specific Protocols
IHC protocol for C10orf104 antibody 26324-1-APDownload protocol
IF protocol for C10orf104 antibody 26324-1-APDownload protocol
IP protocol for C10orf104 antibody 26324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols