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验证数据展示

  • WB analysis of rat liver using 83741-3-RR

    WB analysis of rat liver using 83741-3-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 83741-3-RR

    IHC staining of rat liver using 83741-3-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 83741-3-RR

    IHC staining of rat liver using 83741-3-RR

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83741-3-RR (C-Reactive Protein/CRP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83741-3-RR

    Affinity and Kinetic Characterization of 83741-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83741-3-RR against Rat Crp were performed. The affinity constant is below 1 pM.

C-Reactive Protein/CRP Recombinant antibody

C-Reactive Protein/CRP Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83741-3-RR

产品说明书

CloneNo. 240759B8

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, ELISA

别名

PTX1, PTX 1, CRP, 240759B8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83741-3-RR targets C-Reactive Protein/CRP in WB, IHC, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 C-reactive protein, pentraxin-related
别名 PTX1, PTX 1, CRP, 240759B8
计算分子量25 kDa
观测分子量 25 kDa
GenBank蛋白编号NM_017096.3
基因名称 Crp
Gene ID (NCBI) 25419
RRIDAB_3671339
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP48199
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein belonging to the pentraxin family. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

实验方案

Product Specific Protocols
WB protocol for C-Reactive Protein/CRP antibody 83741-3-RRDownload protocol
IHC protocol for C-Reactive Protein/CRP antibody 83741-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols