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验证数据展示

  • FC experiment of Transfected CHO using 98867-1-RR

    FC experiment of Transfected CHO using 98867-1-RR

    1x10^6 CHO cells or C-Peptide transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human C-Peptide Rabbit RecAb (98867-1-RR, Clone: 253514D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IHC staining of human pancreas using 98867-1-RR

    IHC staining of human pancreas using 98867-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98867-1-RR (C-Peptide antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Insulin antibody included
  • IHC staining of human pancreas using 98867-1-RR

    IHC staining of human pancreas using 98867-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 98867-1-RR (C-Peptide antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Insulin antibody included

Anti-Human C-peptide Rabbit Recombinant Antibody

C-peptide Uni-rAb® Recombinant Antibody for IHC, FC (Intra)
Cat No. 98867-1-RR

产品说明书

CloneNo. 253514D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC (Intra)

别名

C peptide, INS C-Peptide, Insulin C-Peptide

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inTransfected CHO cells
Planning an IHC experiment? We recommend our IHCeasy Insulin Ready-To-Use IHC Kit. Insulin primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98867-1-RR targets C-peptide in IHC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IHC, FC (Intra) Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 INS
别名 C peptide, INS C-Peptide, Insulin C-Peptide
计算分子量110aa, 12 kDa
GenBank蛋白编号NM_000207
基因名称 INS
Gene ID (NCBI) 3630
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP01308
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

C-peptide (connecting peptide) is a short 31-amino-acid polypeptide that connects insulin's A-chain to its B-chain in the proinsulin molecule. It is cleaved from proinsulin in the pancreatic beta cells, and one molecule of C-peptide is released for every molecule of insulin (PMID: 6759081). C-peptide is secreted at a relatively steady rate over long duration, making it an effective biomarker for endogenous insulin secretion (PMID: 41694179).

实验方案

Product Specific Protocols
FC protocol for C-peptide antibody 98867-1-RRDownload protocol
IHC protocol for C-peptide antibody 98867-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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