验证数据展示
经过测试的应用
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas tissue |
Planning an IHC experiment? We recommend our IHCeasy Insulin Ready-To-Use IHC Kit. Insulin primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87566-3-RR targets C-Peptide in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | INS |
| 别名 | INS, insulin |
| 计算分子量 | 110aa, 12 kDa |
| GenBank蛋白编号 | NM_000207 |
| 基因名称 | INS |
| Gene ID (NCBI) | 3630 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01308 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C‑peptide is a 31‑amino‑acid peptide that connects the A and B chains of proinsulin and is co‑secreted with insulin in equimolar amounts from pancreatic beta cells. With a longer plasma half‑life than insulin, it serves as a reliable biomarker of endogenous insulin secretion. Clinically, measuring C‑peptide is crucial for distinguishing type 1 from type 2 diabetes, diagnosing hypoglycemia, and assessing residual beta‑cell function. Emerging evidence also suggests that C‑peptide may exert its own biological effects on vascular and renal tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for C-Peptide antibody 87566-3-RR | Download protocol |
| IHC protocol for C-Peptide antibody 87566-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
