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Validation Data Gallery

  • IF Staining using Biotin-TSA Reagent PR30029. FFPE mouse brain tissue was stained with HRP conjugated GFAP monoclonal antibody at 0.5 μg/mL followed by signal amplification using with Biotin-TSA Reagent PR30029 for 10 minutes at room temperature. Streptavidin-CoraLite® Plus 647 Conjugate (Cat.NO. CL647-PF00030) was diluted at 1:500 and reacted for 10 minutes at room temperature for detection. Cell nucleus (blue) was stained with DAPI.

    IF Staining using Biotin-TSA Reagent PR30029. FFPE mouse brain tissue was stained with HRP conjugated GFAP monoclonal antibody at 0.5 μg/mL followed by signal amplification using with Biotin-TSA Reagent PR30029 for 10 minutes at room temperature. Streptavidin-CoraLite® Plus 647 Conjugate (Cat.NO. CL647-PF00030) was diluted at 1:500 and reacted for 10 minutes at room temperature for detection. Cell nucleus (blue) was stained with DAPI.

  • IF Staining using Biotin-TSA Reagent PR30029. FFPE mouse brain tissue was stained with HRP conjugated TDP43 rabbit recombinant antibody at 0.5 μg/mL followed by signal amplification using with Biotin-TSA Reagent PR30029 for 10 minutes at room temperature. Streptavidin-CoraLite® Plus 647 Conjugate (Cat.NO. CL647-PF00030) was diluted at 1:500 and reacted for 10 minutes at room temperature for detection. Cell nucleus (blue) was stained with DAPI.

    IF Staining using Biotin-TSA Reagent PR30029. FFPE mouse brain tissue was stained with HRP conjugated TDP43 rabbit recombinant antibody at 0.5 μg/mL followed by signal amplification using with Biotin-TSA Reagent PR30029 for 10 minutes at room temperature. Streptavidin-CoraLite® Plus 647 Conjugate (Cat.NO. CL647-PF00030) was diluted at 1:500 and reacted for 10 minutes at room temperature for detection. Cell nucleus (blue) was stained with DAPI.

Biotin-TSA

Cat No. PR30029

应用

多重免疫荧光、原位杂交等实验

特性

酪胺信号放大技术，实现荧光或酶信号放大

偶联物

Biotin

规格价格库存

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产品信息

TSA（Tyramide Signal Amplification）染料是一类基于酪胺信号放大技术的荧光标记试剂。在HRP酶催化下，TSA染料可以与HRP附近蛋白质上的酪氨酸反应形成共价结合并积累，从而可实现信号放大。相比使用直标荧光的一抗或使用荧光二抗进行间接法检测，使用TSA系统可将信号提高数倍至上百倍，加之其与样本形成的共价键稳定不易掉落，因而广泛应用于多重免疫荧光、原位杂交等实验。

Biotin-TSA与Streptavidin荧光标记物联合使用，可以将HRP体系切换至荧光体系而实现更强的荧光信号，Biotin-TSA与Streptavidin HRP标记物联合使用，则可以实现HRP体系的增强。

本产品包含100 uL Biotin-TSA和5 mL扩增液，具体可以使用的次数根据使用场景差异巨大。实验中需要的其他试剂请自行准备或另购。

保存条件

2-8℃保存，有效期12个月。

使用方法

本产品是开放试剂，适合用户自行建立和优化实验方案。

通常的使用思路：

1. 在HRP标记物反应即将结束时，按需配制Biotin-TSA工作液：通常Biotin-TSA与扩增液按1 : 50比例混合，如信号过强，可以调整稀释比例（减少Biotin-TSA用量）。

2. HRP标记物反应结束后进行洗涤。然后加上Biotin-TSA工作液，室温反应10分钟左右（具体可根据需要自行调整反应时间）。

3. 反应结束后洗涤除去Biotin-TSA工作液，加入Streptavidin荧光标记物或酶标记物进行信号放大。具体反应条件请自行摸索。

4. 洗涤并观察结果。

注意事项

1. 使用前请充分理解反应原理再设计实验。如果有疑问请与Proteintech技术支持联系。

2. 部分实验计划原理上合理，但实际实验中可能与预期不符，不代表本产品有质量问题，建议设计不同对照实验加以论证。

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