验证数据展示
经过测试的应用
| Positive IHC detected in | mouse skeletal muscle tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20277-1-AP targets Osteocalcin in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | mouse |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | bone gamma carboxyglutamate protein 1 |
| 别名 | Bglap1, Bglap, OSTCN, Gamma-carboxyglutamic acid-containing protein, Bone Gla protein |
| 计算分子量 | 11 kDa |
| GenBank蛋白编号 | NM_007541 |
| 基因名称 | Osteocalcin |
| Gene ID (NCBI) | 12096 |
| RRID | AB_11232596 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P86546 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Osteocalcin (Bglap1), also named as Bglap2, Bglap, BGP, OC, OG1 and mOC-A, belongs to the osteocalcin/matrix Gla protein family. Bglap1 constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. The antibody is specific to mouse Bglap1.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Osteocalcin antibody 20277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy | ||
Acta Biomater A composite membrane with microtopographical morphology to regulate cellular behavior for improved tissue regeneration | ||
Sci Rep Experimental arthritis and Porphyromonas gingivalis administration synergistically decrease bone regeneration in femoral cortical defects. | ||
J Dent Sci Orthodontic tension promotes cementoblast mineralization by regulating autophagy | ||
Phytomedicine Single-cell RNA transcriptomics reveals Du-Zhong-Wan promotes osteoporotic fracture healing via YAP/β-catenin/VEGF axis in BMSCs | ||
Mol Med Rep LPS‑mediated adaptation accelerates ecto‑MSCs differentiation into osteoblasts |
