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验证数据展示

  • WB analysis using 83706-6-RR

    WB analysis using 83706-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83706-6-RR (Beta galactosidase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 83706-6-RR

    WB analysis of A549 using 83706-6-RR

    WB result of Beta galactosidase antibody (83706-6-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta galactosidase transfected A549 cells.

  • IF Staining of HepG2 using 83706-6-RR

    IF Staining of HepG2 using 83706-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Beta galactosidase antibody (83706-6-RR, Clone: 240788F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A549 using 83706-6-RR

    FC experiment of A549 using 83706-6-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Beta galactosidase Recombinant antibody (83706-6-RR, Clone:240788F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83706-6-RR

    Affinity and Kinetic Characterization of 83706-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83706-6-RR against Human Beta galactosidase were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

Beta galactosidase Recombinant monoclonal antibody

Beta galactosidase Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83706-6-RR

产品说明书

CloneNo. 240788F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

GLB1, 240788F1, Acid beta-galactosidase, Beta-galactosidase, EBP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, SH-SY5Y cells, HEK-293 cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83706-6-RR targets Beta galactosidase in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7792

Product name: Recombinant human Beta galactosidase protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 329-677 aa of BC007493

Sequence: TSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 galactosidase, beta 1
别名 GLB1, 240788F1, Acid beta-galactosidase, Beta-galactosidase, EBP
计算分子量 76 kDa
观测分子量 62-67 kDa
GenBank蛋白编号BC007493
基因名称 GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
RRIDAB_3671308
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP16278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.

实验方案

Product Specific Protocols
FC protocol for Beta galactosidase antibody 83706-6-RRDownload protocol
IF protocol for Beta galactosidase antibody 83706-6-RRDownload protocol
WB protocol for Beta galactosidase antibody 83706-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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