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验证数据展示

  • WB analysis of SH-SY5Y using 15518-1-AP (same clone as 15518-1-PBS)

    WB analysis of SH-SY5Y using 15518-1-AP (same clone as 15518-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

    IHC staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

    IHC staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15518-1-AP (same clone as 15518-1-PBS)

    IF Staining of HeLa using 15518-1-AP (same clone as 15518-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

  • IF Staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

    IF Staining of human prostate cancer using 15518-1-AP (same clone as 15518-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human prostate cancer tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

    IF Staining of human liver cancer using 15518-1-AP (same clone as 15518-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15518-1-PBS in a different storage buffer formulation.

Beta Galactosidase Polyclonal antibody, PBS Only

Beta Galactosidase Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 15518-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

GLB1, Acid beta-galactosidase, Beta-galactosidase, EBP, EC:3.2.1.23

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

15518-1-PBS targets Beta Galactosidase in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Beta Galactosidase fusion protein Ag7792 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 galactosidase, beta 1
别名 GLB1, Acid beta-galactosidase, Beta-galactosidase, EBP, EC:3.2.1.23
计算分子量 76 kDa
观测分子量 67 kDa
GenBank蛋白编号BC007493
基因名称 GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
RRIDAB_2263448
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16278
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.