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验证数据展示

  • WB analysis using 81115-1-RR (same clone as 81115-1-PBS)

    WB analysis using 81115-1-RR (same clone as 81115-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81115-1-RR (beta actin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

  • IHC staining of rat colon using 81115-1-RR (same clone as 81115-1-PBS)

    IHC staining of rat colon using 81115-1-RR (same clone as 81115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 81115-1-RR (Beta Actin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTB antibody included
  • IHC staining of rat colon using 81115-1-RR (same clone as 81115-1-PBS)

    IHC staining of rat colon using 81115-1-RR (same clone as 81115-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 81115-1-RR (Beta Actin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTB antibody included
  • IF Staining of U2OS using 81115-1-RR (same clone as 81115-1-PBS)

    IF Staining of U2OS using 81115-1-RR (same clone as 81115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using beta actin antibody (81115-1-RR, Clone: 4H1 ) at dilution of 1:1900 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 81115-1-RR (same clone as 81115-1-PBS)

    IF Staining of MDCK using 81115-1-RR (same clone as 81115-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using Beta Actin antibody (81115-1-RR, Clone: 4H1 ) at dilution of 1:4000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81115-1-PBS in a different storage buffer formulation.

Beta Actin Recombinant antibody, PBS Only

Beta Actin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81115-1-PBS

产品说明书

CloneNo. 4H1

宿主/亚型

Rabbit / IgG

种属反应性

Human, pig, rat, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.

产品信息

81115-1-PBS targets Beta Actin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, pig, rat, mouse
免疫原 Beta Actin fusion protein Ag14521 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actin, beta
别名 ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin
计算分子量 375 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC002409
基因名称 Beta Actin
Gene ID (NCBI) 60
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP60709
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )