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验证数据展示

  • WB analysis using 33133-1-AP

    WB analysis using 33133-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33133-1-AP (BXDC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 33133-1-AP

    IF Staining of U2OS using 33133-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BXDC5 antibody (33133-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

BXDC5 Polyclonal antibody

BXDC5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 33133-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Brix domain-containing protein 5, Ribosome biogenesis protein RPF1, Ribosome production factor 1, Ribosome Production Factor 1 Homolog, RPF1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, U-251 cells, RKO cells, RT-4 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33133-1-AP targets BXDC5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag37505

Product name: Recombinant human BXDC5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-349 aa of BC016051

Sequence: MAKAGDKSGSSGKKSLKRKAAAEELQEAAGAGDGATENGVQPPKAAAFPPGFSISEIKNKQRRHLMFTRWKQQQRKEKLAAKKKLKKEREALGDKAPPKPVPKTIDNQRVYDETTVDPNDEEVAYDEATDEFASYFNKQTSPKILITTSDRPHGRTVRLCEQLSTVIPNSHVYYRRGLALKKIIPQCIARDFTDLIVINEDRKTPNGLILSHLPNGPTAHFKMSSVRLRKEIKRRGKDPTEHIPEIILNNFTTRLGHSIGRMFASLFPHNPQFIGRQVATFHNQRDYIFFRFHRYIFRSEKKVGIQELGPRFTLKLRSLQKGTFDSKYGEYEWVHKPREMDTSRRKFHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 brix domain containing 5
别名 Brix domain-containing protein 5, Ribosome biogenesis protein RPF1, Ribosome production factor 1, Ribosome Production Factor 1 Homolog, RPF1
计算分子量 349 aa, 40 kDa
观测分子量48 kDa
GenBank蛋白编号BC016051
基因名称 BXDC5
Gene ID (NCBI) 80135
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H9Y2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BXDC5 (Brix domain-containing protein 5), also known as ribosome biogenesis protein RPF1, is a protein containing a Brix domain and is localized in the nucleolus. The Brix domain is a region that shares homology with the yeast protein Pitx1 (ribosome biogenesis protein BRX1). BXDC5 is a key factor in ribosome biogenesis, participating in the maturation of the 60S ribosomal subunit. This protein exerts its function by binding to specific rRNA structures and acts as a downstream effector molecule of the Ran signaling pathway, involved in ribosome biogenesis. In colorectal cancer (CRC), BXDC5 influences the occurrence and development of tumors by regulating ribosome biogenesis.

实验方案

Product Specific Protocols
IF protocol for BXDC5 antibody 33133-1-APDownload protocol
WB protocol for BXDC5 antibody 33133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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