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验证数据展示

  • WB analysis using 17584-1-AP

    WB analysis using 17584-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17584-1-AP (BXDC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17584-1-AP

    IF Staining of HeLa using 17584-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BXDC1 antibody (17584-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

BXDC1 Polyclonal antibody

BXDC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17584-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

RPF2, Brix domain-containing protein 1, brix domain containing 1, bA397G5.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HL-60 cells, HEK-293T cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17584-1-AP targets BXDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11268

Product name: Recombinant human BXDC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-297 aa of BC035314

Sequence: MDTLDRVVKPKTKRAKRFLEKREPKLNENIKNAMLIKGGNANATVTKVLKDVYALKKPYGVLYKKKNITRPFEDQTSLEFFSKKSDCSLFMFGSHNKKRPNNLVIGRMYDYHVLDMIELGIENFVSLKDIKNSKCPEGTKPMLIFAGDDFDVTEDYRRLKSLLIDFFRGPTVSNIRLAGLEYVLHFTALNGKIYFRSYKLLLKKSGCRTPRIELEEMGPSLDLVLRRTHLASDDLYKLSMKMPKALKPKKKKNISHDTFGTTYGRIHMQKQDLSKLQTRKMKGLKKRPAERITEDHE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 brix domain containing 1
别名 RPF2, Brix domain-containing protein 1, brix domain containing 1, bA397G5.4
计算分子量 306 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC035314
基因名称 BXDC1
Gene ID (NCBI) 84154
RRIDAB_1939419
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7B2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BXDC1 antibody 17584-1-APDownload protocol
IF protocol for BXDC1 antibody 17584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols