验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, hESC cells, human brain tissue, Jurkat cells, mouse heart tissue, mouse liver tissue, mouse lung tissue, Raji cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11798-1-AP targets BUD31 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | BUD31 fusion protein Ag2373 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | BUD31 homolog (S. cerevisiae) |
| 别名 | BUD31, BUD31 homolog (S. cerevisiae), EDG 2, EDG2, fSAP17, G10, Protein BUD31 homolog, Protein EDG 2, Protein G10 homolog, YCR063W |
| 计算分子量 | 144 aa, 17 kDa |
| 观测分子量 | 17-20 kDa |
| GenBank蛋白编号 | BC022821 |
| 基因名称 | BUD31 |
| Gene ID (NCBI) | 8896 |
| RRID | AB_2274894 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41223 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BUD31 antibody 11798-1-AP | Download protocol |
| IHC protocol for BUD31 antibody 11798-1-AP | Download protocol |
| IP protocol for BUD31 antibody 11798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. | ||
Cell Death Differ Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation
| ||
Nat Commun Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12
| ||
Clin Exp Med The role of BUD31 in clear cell renal cell carcinoma: prognostic significance, alternative splicing, and tumor immune environment
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