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BUD13 Polyclonal antibody

BUD13 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20163-1-AP

Print datasheet

Synonyms

BUD13, BUD13 homolog, BUD13 homolog (S. cerevisiae), fSAP71

验证数据展示

  • WB analysis using 20163-1-AP

    WB analysis using 20163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20163-1-AP (BUD13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20163-1-AP

    IHC staining of mouse testis using 20163-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20163-1-AP (BUD13 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 20163-1-AP

    IHC staining of mouse testis using 20163-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20163-1-AP (BUD13 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, PC-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20163-1-AP targets BUD13 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen BUD13 fusion protein Ag13665 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name BUD13 homolog (S. cerevisiae)
Synonyms BUD13, BUD13 homolog, BUD13 homolog (S. cerevisiae), fSAP71
Calculated Molecular Weight 619 aa, 71 kDa
Observed Molecular Weight 71-85 kDa
GenBank Accession NumberBC006350
Gene Symbol BUD13
Gene ID (NCBI) 84811
RRIDAB_2878650
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRD0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BUD13 homolog (BUD13) is a component of the retention and splicing (RES) complex which was previously identified in yeast as a splicing factor affecting nuclear pre-mRNA retention. As an RNA binding protein (RBP), BUD13 expression is associated with poor prognosis and may be involved in the development and progression of hepatocellular carcinoma (PMID: 37605593).

实验方案

Product Specific Protocols
WB protocol for BUD13 antibody 20163-1-APDownload protocol
IHC protocol for BUD13 antibody 20163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols