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验证数据展示

  • WB analysis using 83266-5-RR (same clone as 83266-5-PBS)

    WB analysis using 83266-5-RR (same clone as 83266-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83266-5-RR (BUB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    FC experiment of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BUB3 Recombinant antibody (83266-5-RR, Clone:240087D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83266-5-RR

    Affinity and Kinetic Characterization of 83266-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83266-5-RR against Human BUB3 were performed. The affinity constant is 0.228 nM.

BUB3 Recombinant monoclonal antibody, PBS Only

BUB3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83266-5-PBS

产品说明书

CloneNo. 240087D9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

hBUB3, BUB3L, BUB 3, 240087D9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BUB3 Ready-To-Use IHC Kit. BUB3 primary antibody included.

产品信息

83266-5-PBS targets BUB3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag25608

Product name: Recombinant human BUB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 205-328 aa of BC005138

Sequence: VEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 3 homolog (yeast)
别名 hBUB3, BUB3L, BUB 3, 240087D9
计算分子量 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC005138
基因名称 BUB3
Gene ID (NCBI) 9184
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO43684
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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