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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 27073-1-AP

    WB analysis using 27073-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27073-1-AP (BUB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 27073-1-AP

    IHC staining of human pancreas cancer using 27073-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27073-1-AP (BUB3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BUB3 antibody included

BUB3 Polyclonal antibody

BUB3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27073-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

BUB3, BUB3L, hBUB3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, MCF-7 cells, C6 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy BUB3 Ready-To-Use IHC Kit. BUB3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

27073-1-AP targets BUB3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原

CatNo: Ag25608

Product name: Recombinant human BUB3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 205-328 aa of BC005138

Sequence: VEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 3 homolog (yeast)
别名 BUB3, BUB3L, hBUB3
计算分子量 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC005138
基因名称 BUB3
Gene ID (NCBI) 9184
RRIDAB_2880743
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43684
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BUB3 antibody 27073-1-APDownload protocol
IHC protocol for BUB3 antibody 27073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
View Article
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
View Article
humanWB

Cancer Gene Ther

Pan-cancer oncogenic properties and therapeutic potential of SF3B4

Authors - Yanmei Shi
View Article
humanWB

J Oncol

CDCA3 Predicts Poor Prognosis and Affects CD8+ T Cell Infiltration in Renal Cell Carcinoma

Authors - Yuanyuan Bai
View Article
humanWB

J Biomed Sci

BUB1B monoallelic germline variants contribute to prostate cancer predisposition by triggering chromosomal instability

Authors - Maria P Silva
View Article