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BUB3 Polyclonal antibody

BUB3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27073-1-AP

Print datasheet

Synonyms

BUB3, BUB3L, hBUB3

验证数据展示

  • WB analysis using 27073-1-AP

    WB analysis using 27073-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27073-1-AP (BUB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27073-1-AP

    IHC staining of human colon using 27073-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27073-1-AP (BUB3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 27073-1-AP

    IHC staining of human colon using 27073-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27073-1-AP (BUB3 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293T cells, MCF-7 cells, C6 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

27073-1-AP targets BUB3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen BUB3 fusion protein Ag25608 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Synonyms BUB3, BUB3L, hBUB3
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC005138
Gene Symbol BUB3
Gene ID (NCBI) 9184
RRIDAB_2880743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BUB3 antibody 27073-1-APDownload protocol
IHC protocol for BUB3 antibody 27073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
View Article
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
View Article
humanWB

J Oncol

CDCA3 Predicts Poor Prognosis and Affects CD8+ T Cell Infiltration in Renal Cell Carcinoma

Authors - Yuanyuan Bai
View Article