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BubR1 Polyclonal antibody
BubR1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, K-562 cells, HeLa cells, human testis tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SKOV-3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11504-2-AP targets BubR1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | BubR1 fusion protein Ag2053 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | budding uninhibited by benzimidazoles 1 homolog beta (yeast) |
Synonyms | Bub1A, BUB1B, BUB1beta, BubR1, hBUBR1, MAD3L, Protein SSK1, SSK1 |
Calculated Molecular Weight | 1050 aa, 120 kDa |
Observed Molecular Weight | 120-130 kDa |
GenBank Accession Number | BC018739 |
Gene Symbol | BUB1B |
Gene ID (NCBI) | 701 |
RRID | AB_2066073 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60566 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for BubR1 antibody 11504-2-AP | Download protocol |
IHC protocol for BubR1 antibody 11504-2-AP | Download protocol |
IF protocol for BubR1 antibody 11504-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. | ||
Cancer Lett Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells. | ||
Bioorg Chem Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors. | ||
Eur J Med Chem Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents. | ||
Int J Biol Sci The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. |