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验证数据展示

  • WB analysis using 86843-4-RR (same clone as 86843-4-PBS)

    WB analysis using 86843-4-RR (same clone as 86843-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86843-4-RR (BUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86843-4-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 86843-4-RR (same clone as 86843-4-PBS)

    WB analysis of U2OS using 86843-4-RR (same clone as 86843-4-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 86843-4-RR (BUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86843-4-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 86843-4-RR (same clone as 86843-4-PBS)

    WB analysis of K-562 using 86843-4-RR (same clone as 86843-4-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 86843-4-RR (BUB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86843-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02812-1

    Sandwich ELISA sample test of MP02812-1

    The mean BUB1 concentration was determined to be 304.97 ng/mL in MKN-45 cell extract based on a 2.0 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02812-1

    Sandwich ELISA standard curve of MP02812-1

    Sandwich ELISA standard curve of MP02812-1, Human BUB1 Recombinant Matched Antibody Pair - PBS only. 86843-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4138. 86843-4-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

  • IF Staining of HepG2 using 86843-4-RR (same clone as 86843-4-PBS)

    IF Staining of HepG2 using 86843-4-RR (same clone as 86843-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BUB1 antibody (86843-4-RR, Clone: 251580E5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86843-4-PBS in a different storage buffer formulation.

BUB1 Recombinant monoclonal antibody, PBS Only (Detector)

BUB1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 86843-4-PBS

产品说明书

CloneNo. 251580E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

BUB1A, BUB1L, EC:2.7.11.1, hBUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86843-4-PBS targets BUB1 as part of a matched antibody pair:

MP02812-1: 86843-5-PBS capture and 86843-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4138

Product name: Recombinant human BUB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 785-1083 aa of BC028201

Sequence: KLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHDCEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKPEGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLLRQKLKKVFQQHYTNKIRALRNRLIVLLLECKRS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 1 homolog (yeast)
别名 BUB1A, BUB1L, EC:2.7.11.1, hBUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1
计算分子量 1085 aa, 122 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC028201
基因名称 BUB1
Gene ID (NCBI) 699
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43683
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).

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