验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24959-1-AP targets BTNL8 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag21203 Product name: Recombinant human BTNL8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 90-147 aa of BC119696 Sequence: CSMRHAHLSREVESRVQIGDTFFKPISWHLATKVLGILCCGLFFGIVGLKIFFSKFQW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | butyrophilin-like 8 |
| 别名 | butyrophilin like 8, Butyrophilin like protein 8, Butyrophilin-like protein 8, UNQ702/PRO1347 |
| 计算分子量 | 500 aa, 57 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC119696 |
| 基因名称 | BTNL8 |
| Gene ID (NCBI) | 79908 |
| RRID | AB_2879822 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UX41 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BTNL8 (butyrophilin-like 8) belongs to the butyrophilin (BTN) family, a group of immunoglobulin superfamily members structurally and functionally related to the B7 family of immune costimulatory molecules. The protein is predicted to localize to the plasma membrane and is involved in T cell receptor signaling and regulation of cytokine production. It is highly expressed in the gastrointestinal tract, particularly in the duodenum and small intestine, as well as in the colon and rectum.BTNL8 is also expressed in neutrophils. Functionally, BTNL8 plays a role in modulating immune responses. It can augment antigen-induced activation of T lymphocytes, promoting T-cell proliferation and cytokine production.(PMID:24036152)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for BTNL8 antibody 24959-1-AP | Download protocol |
| WB protocol for BTNL8 antibody 24959-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
