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验证数据展示

  • WB analysis of MCF-7 using 24959-1-AP

    WB analysis of MCF-7 using 24959-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24959-1-AP (BTNL8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 24959-1-AP

    IHC staining of human stomach cancer using 24959-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24959-1-AP (BTNL8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BTNL8 Polyclonal antibody

BTNL8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24959-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

UNQ702/PRO1347, Butyrophilin-like protein 8, Butyrophilin like protein 8, butyrophilin like 8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24959-1-AP targets BTNL8 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 BTNL8 fusion protein Ag21203 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 butyrophilin-like 8
别名 UNQ702/PRO1347, Butyrophilin-like protein 8, Butyrophilin like protein 8, butyrophilin like 8
计算分子量 500 aa, 57 kDa
观测分子量 50 kDa
GenBank蛋白编号BC119696
基因名称 BTNL8
Gene ID (NCBI) 79908
RRIDAB_2879822
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UX41
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTNL8 antibody 24959-1-APDownload protocol
IHC protocol for BTNL8 antibody 24959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols