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验证数据展示

  • WB analysis of Jurkat using 15896-1-AP

    WB analysis of Jurkat using 15896-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15896-1-AP (BTN3A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15896-1-AP

    IF Staining of HepG2 using 15896-1-AP

    Immunofluorescent analysis of HepG2 cells, using BTN3A3 antibody 15896-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of Jurkat using 15896-1-AP

    IP experiment of Jurkat using 15896-1-AP

    IP result of anti-BTN3A3 (IP:15896-1-AP, 4ug; Detection:15896-1-AP 1:500) with Jurkat cells lysate 4000ug.

BTN3A3 Polyclonal antibody

BTN3A3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 15896-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

BTF3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, U-937 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15896-1-AP targets BTN3A3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 BTN3A3 fusion protein Ag8711 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 butyrophilin, subfamily 3, member A3
别名 BTF3
计算分子量 584 aa, 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC015815
基因名称 BTN3A3
Gene ID (NCBI) 10384
RRIDAB_2065784
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00478
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTN3A3 antibody 15896-1-APDownload protocol
IF protocol for BTN3A3 antibody 15896-1-APDownload protocol
IP protocol for BTN3A3 antibody 15896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols