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验证数据展示

  • WB analysis of SKOV-3 using 15148-1-AP

    WB analysis of SKOV-3 using 15148-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15148-1-AP (BTN3A2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of SKOV-3 using 15148-1-AP

    WB analysis of SKOV-3 using 15148-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 15148-1-AP (BTN3A2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

BTN3A2 Polyclonal antibody

BTN3A2 Polyclonal Antibody for WB, ELISA
Cat No. 15148-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

BT3.2, BT3.3, BTF3, BTF4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSKOV-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15148-1-AP targets BTN3A2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2212

Product name: Recombinant human BTN3A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3-334 aa of BC020214

Sequence: MASSLAFLLLNFHVSLLLVQLLTPCSAQFSVLGPSGPILAMVGEDADLPCHLFPTMSAETMELKWVSSSLRQVVNVYADGKEVEDRQSAPYRGRTSILRDGITAGKAALRIHNVTASDSGKYLCYFQDGDFYEKALVELKVAALGSNLHVEVKGYEDGGIHLECRSTGWYPQPQIQWSNAKGENIPAVEAPVVADGVGLYEVAASVIMRGGSGEGVSCIIRNSLLGLEKTASISIADPFFRSAQPWIAALAGTLPILLLLLAGASYFLWRQQKEITALSSEIESEQEMKEMGYAATEREISLRESLQEELKRKKIQYLTRGEESSSDTNKSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 butyrophilin, subfamily 3, member A2
别名 BT3.2, BT3.3, BTF3, BTF4
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC020214
基因名称 BTN3A2
Gene ID (NCBI) 11118
RRIDAB_2878112
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78410
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BTN3A2, also named as Butyrophilin subfamily 3 member A2, is a 334 amino acid protein, which contains 1 Ig-like V-type (immunoglobulin-like) domain and belongs to the immunoglobulin superfamily. BTN3A2 plays a role in T-cell responses in the adaptive immune response and inhibits the release of IFNG from activated T-cells. BTN3A2 can form Homodimer and is detected in T-cells and natural killer cells.

实验方案

Product Specific Protocols
WB protocol for BTN3A2 antibody 15148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cancer Immunol Res

RIPK3 Orchestrates Fatty Acid Metabolism in Tumor-Associated Macrophages and Hepatocarcinogenesis.

Authors - Lei Wu
View Article
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