BTK Polyclonal antibody

BTK Polyclonal Antibody for WB, IP, ELISA
Cat No. 21581-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA and More (1)

EC:2.7.10.2, BPK, B-cell progenitor kinase, ATK, AGMX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inDaudi cells, Ramos cells, HT-29 cells, NIH/3T3 cells
Positive IP detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy BTK Ready-To-Use IHC Kit. BTK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21581-1-AP targets BTK in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原

CatNo: Ag16133

Product name: Recombinant human BTK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 28-184 aa of BC109079

Sequence: RLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Bruton agammaglobulinemia tyrosine kinase
别名 EC:2.7.10.2, BPK, B-cell progenitor kinase, ATK, AGMX1
计算分子量 659 aa, 76 kDa
观测分子量 70-76 kDa
GenBank蛋白编号BC109079
基因名称 BTK
Gene ID (NCBI) 695
RRIDAB_10734434
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ06187
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTK antibody 21581-1-APDownload protocol
IP protocol for BTK antibody 21581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction.

Authors - Weijia Sun
humanWB

Int J Mol Med

Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus.

Authors - Linlin Sheng
mouseWB

Redox Biol

CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway

Authors - Chengli Liu
humanWB,IHC

J Cancer

Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma

Authors - Yan Wang
humanWB

Cell Death Discov

Tuning the B-CLL microenvironment: evidence for BAG3 protein- mediated regulation of stromal fibroblasts activity

Authors - Anna Basile
Loading...