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BTK Polyclonal antibody

BTK Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21581-1-AP

Print datasheet

Synonyms

AGMX1, AT, ATK, B cell progenitor kinase, BPK, Bruton tyrosine kinase, BTK, IMD1, PSCTK1, Tyrosine protein kinase BTK, XLA

验证数据展示

  • WB analysis using 21581-1-AP

    WB analysis using 21581-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21581-1-AP (BTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse spleen using 21581-1-AP

    IP experiment of mouse spleen using 21581-1-AP

    IP result of anti-BTK (IP:21581-1-AP, 4ug; Detection:21581-1-AP 1:300) with mouse spleen tissue lysate 4800ug.

经过测试的应用

Positive WB detected inDaudi cells, Ramos cells, HT-29 cells, NIH/3T3 cells
Positive IP detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy BTK Ready-To-Use IHC Kit. BTK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

21581-1-AP targets BTK in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen BTK fusion protein Ag16133 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Bruton agammaglobulinemia tyrosine kinase
Synonyms AGMX1, AT, ATK, B cell progenitor kinase, BPK, Bruton tyrosine kinase, BTK, IMD1, PSCTK1, Tyrosine protein kinase BTK, XLA
Calculated Molecular Weight 659 aa, 76 kDa
Observed Molecular Weight 70-76 kDa
GenBank Accession NumberBC109079
Gene Symbol BTK
Gene ID (NCBI) 695
RRIDAB_10734434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06187
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BTK antibody 21581-1-APDownload protocol
IP protocol for BTK antibody 21581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction.

Authors - Weijia Sun
View Article
humanWB

Int J Mol Med

Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus.

Authors - Linlin Sheng
View Article
mouseWB

Redox Biol

CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway

Authors - Chengli Liu
View Article