验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, Ramos cells, HT-29 cells, NIH/3T3 cells |
| Positive IP detected in | mouse spleen tissue |
Planning an IHC experiment? We recommend our IHCeasy BTK Ready-To-Use IHC Kit. BTK primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
21581-1-AP targets BTK in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16133 Product name: Recombinant human BTK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-184 aa of BC109079 Sequence: RLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Bruton agammaglobulinemia tyrosine kinase |
| 别名 | EC:2.7.10.2, BPK, B-cell progenitor kinase, ATK, AGMX1 |
| 计算分子量 | 659 aa, 76 kDa |
| 观测分子量 | 70-76 kDa |
| GenBank蛋白编号 | BC109079 |
| 基因名称 | BTK |
| Gene ID (NCBI) | 695 |
| RRID | AB_10734434 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q06187 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for BTK antibody 21581-1-AP | Download protocol |
| WB protocol for BTK antibody 21581-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Anoctamin 1 controls bone resorption by coupling Cl- channel activation with RANKL-RANK signaling transduction. | ||
Int J Mol Med Overexpression of FcγRIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. | ||
Redox Biol CXCR4-BTK axis mediate pyroptosis and lipid peroxidation in early brain injury after subarachnoid hemorrhage via NLRP3 inflammasome and NF-κB pathway | ||
J Cancer Identification and validation of a novel apoptosis-related prognostic risk score model for lung adenocarcinoma | ||
Cell Death Discov Tuning the B-CLL microenvironment: evidence for BAG3 protein- mediated regulation of stromal fibroblasts activity |
